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Expression analysis of human EGF R on HEK293/Human EGF R Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human EGF R Stable Cell Line or negative control cell using PE-labeled anti-human EGF R antibody.
5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human EGF R, His Tag (Cat. No. EGR-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).
Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R Protein, His Tag (Cat.No. EGR-H522a) with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).
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